Journal: Biosensors

Article Title: Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI).

doi: 10.3390/bios13040455

Figure Lengend Snippet: Figure 1. Simplified biosensor principle: The cTnI–antibody–peroxidase conjugate binds to the analyte cTnI (PDB 4Y99) and cannot interact with the peptide-functionalized column (coated with a peptide–BSA conjugate). The intensity of the chemiluminescence produced by the enzyme–substrate reaction is largely proportional to the analyte concentration in the sample.

Article Snippet: After another wash with PBST, 100 μL of the cTnI calibrator (recombinant cTnI from HyTest Ltd.) and the expressed cTnI (both diluted in LowCross-Buffer, Candor Bioscience GmbH) were pipetted and incubated for 1 h at room temperature.

Techniques: Produced, Concentration Assay

Journal: Biosensors

Article Title: Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI).

doi: 10.3390/bios13040455

Figure Lengend Snippet: Figure 4. Microfluidic setup for the detection of cardiac troponin I (cTnI). The sample injection was performed by a 6-way valve and a continuous flow of running buffer and chemiluminescence substrate. After passing the peptide–BSA affinity column, the sample and substrate were combined 1:1 inside a mixer chip. This was followed by an incubation loop for preincubation and the flow cell for optical detection.

Article Snippet: After another wash with PBST, 100 μL of the cTnI calibrator (recombinant cTnI from HyTest Ltd.) and the expressed cTnI (both diluted in LowCross-Buffer, Candor Bioscience GmbH) were pipetted and incubated for 1 h at room temperature.

Techniques: Injection, Incubation

Journal: Biosensors

Article Title: Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI).

doi: 10.3390/bios13040455

Figure Lengend Snippet: Figure 6. Quantification of cTnI expressed in E. coli using commercially available cTnI (Hytest 8RTI7) was used to calibrate the sandwich ELISA, as recommended by the supplier [31].

Article Snippet: After another wash with PBST, 100 μL of the cTnI calibrator (recombinant cTnI from HyTest Ltd.) and the expressed cTnI (both diluted in LowCross-Buffer, Candor Bioscience GmbH) were pipetted and incubated for 1 h at room temperature.

Techniques: Sandwich ELISA

Journal: Biosensors

Article Title: Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI).

doi: 10.3390/bios13040455

Figure Lengend Snippet: Figure 9. Measurement series of cTnI in a concentration range between 0 and 64 µg/L and estimation of the dynamic range (see Figure S13).

Article Snippet: After another wash with PBST, 100 μL of the cTnI calibrator (recombinant cTnI from HyTest Ltd.) and the expressed cTnI (both diluted in LowCross-Buffer, Candor Bioscience GmbH) were pipetted and incubated for 1 h at room temperature.

Techniques: Concentration Assay

Journal: Biosensors

Article Title: Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI).

doi: 10.3390/bios13040455

Figure Lengend Snippet: Figure 12. Measurement of human plasma, diluted 1:40 in running buffer and spiked with rec. cTnI in a range between 0 and 20 µg/L. The dilution factor is not included in the given concentrations.

Article Snippet: After another wash with PBST, 100 μL of the cTnI calibrator (recombinant cTnI from HyTest Ltd.) and the expressed cTnI (both diluted in LowCross-Buffer, Candor Bioscience GmbH) were pipetted and incubated for 1 h at room temperature.

Techniques: Clinical Proteomics

Journal: Biosensors

Article Title: Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI).

doi: 10.3390/bios13040455

Figure Lengend Snippet: Figure 13. Linearity of the cTnI detection in spiked human serum and plasma. Limits of detection (LoD) of 70 and 60 µg/L for serum and plasma, respectively, have been calculated.

Article Snippet: After another wash with PBST, 100 μL of the cTnI calibrator (recombinant cTnI from HyTest Ltd.) and the expressed cTnI (both diluted in LowCross-Buffer, Candor Bioscience GmbH) were pipetted and incubated for 1 h at room temperature.

Techniques: Clinical Proteomics

Journal: Analytical biochemistry

Article Title: Extension of dynamic range of sensitive nanoparticle-based immunoassays.

doi: 10.1016/j.ab.2013.10.034

Figure Lengend Snippet: Fig.2. Dose–response curves for the D-dimer and cTnI immunoassays. The error bars represent the standard deviation measured from three replicate microtitration wells. (A) The D-dimer assay exploiting 8D3F(ab0)2 as the solid-phase and FDP14 as the detection antibody. (j) 0 lg/ml free FDP14/8D3F(ab0)2, (d) 5 lg/ml free 8D3F(ab0)2, (.) 15 lg/ ml free 8D3F(ab0)2, (}) 5 lg/ml free FDP14, and (s) 15 lg/ml free FDP14. (B) The D-dimer assay exploiting FDP14 as the solid-phase and 8D3F(ab0)2 as the detection antibody. (j) 0 lg/ml free DP14/8D3F(ab0)2, (N) 5 lg/ml free 8D3F(ab0)2, (d) 15 lg/ml free 8D3F(ab0)2, (h) 1 lg/ml free FDP14, and (}) 5 lg/ml free FDP14. (C) The cTnI assay exploiting 8I7 as the solid-phase and 9707 as the detection antibody. (h) 0 lg/ml free 8I7/9707, (N) 5 lg/ml free 9707, (.) 10 lg/ml free 9707, (d) 25 lg/ml free 9707, and (}) 10 lg/ml free 8I7. (D) The cTnI assay exploiting 9707 as the solid-phase and 8I7 as the detection antibody. (h) 0 lg/ml free 8I7/9707, (N) 5 lg/ml free 8I7, (.) 10 lg/ml free 8I7, (d) 25 lg/ml free 8I7, and (}) 10 lg/ml free 9707.

Article Snippet: Materials and methods D-dimer and cTnI calibrators and clinical samples The D-dimer calibration material was prepared from partially purified D-dimer from human fibrin digested with human plasmin (Biokit, Barcelona, Spain), and human cTnI (native, tissue-derived cTnI–cardiac troponin T–troponin C complex) was purchased from HyTest (Turku, Finland).

Techniques: Standard Deviation, D-dimer Assay